14MBIB12 - Molecular Diagnostics
Course specification | ||||
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Course title | Molecular Diagnostics | |||
Acronym | 14MBIB12 | |||
Study programme | Biochemical Engineering and Biotechnology | |||
Module | ||||
Lecturer (for classes) | ||||
Lecturer/Associate (for practice) | ||||
Lecturer/Associate (for OTC) | ||||
ESPB | 4.0 | Status | ||
Condition | Microbiology, Biochemistry | Облик условљености | ||
The goal | To gain the knowledge about identification of microorganisms using genetic information and molecular techniques. | |||
The outcome | Theoretical knowledge about the molecular basis for the systematics of organism, particularly those base don the sequence of the 16/18 S rRNA gene, and use of this sequence in the rapid identification of organisms. Theoretical and practical knowledge about molecular techniques that can be used for the analysis of complex microbial ecosystems that can be found in food, water and other biological materials. | |||
Contents | ||||
Contents of lectures | Theoretical knowledge about sysetematics of organisms based on the DNA sequence, and the use of molecular techniques for the analysis of (complex) microbial ecosystems. The main focus of the subject is understanding principles and interpretation of results obtained with the following molecular techniques: isolation of nucleic acids, polymerase chain reaction (PCR), ligation detection reaction (LDR), hybridization of DNA molecules with oligonucleotide probes both on the level of cells, or on the DNA array platforms, molecular techniques for profiling such as denaturant gradient gel electrophoresis (DGGE). The subject will give basis for bioinformatics analysis that include searches in the public databases, analysis and identification of organisms based on the DNA sequence. | |||
Contents of exercises | Practical part of the subject will include laboratory projects, where students will apply some of the molecular techniques that will be applied for the diagnostics of model ecosystems. Bioinformatics exercises will include analysis of the 16S or 18S rRNA sequence using software such as ClustalX and ARB and searches of the public databases. | |||
Literature | ||||
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Number of hours per week during the semester/trimester/year | ||||
Lectures | Exercises | OTC | Study and Research | Other classes |
1 | 1 | 1 | ||
Methods of teaching | Lectures, practical work (laboratory and bioinformatics exercises), essay. | |||
Knowledge score (maximum points 100) | ||||
Pre obligations | Points | Final exam | Points | |
Activites during lectures | Test paper | |||
Practical lessons | 20 | Oral examination | 60 | |
Projects | ||||
Colloquia | ||||
Seminars | 20 |