14MEB - Electrochemical Biosensors
Course specification | ||||
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Course title | Electrochemical Biosensors | |||
Acronym | 14MEB | |||
Study programme | Biochemical Engineering and Biotechnology,Chemical engineering | |||
Module | ||||
Lecturer (for classes) | ||||
Lecturer/Associate (for practice) | ||||
Lecturer/Associate (for OTC) | ||||
ESPB | 4.0 | Status | ||
Condition | none | Облик условљености | ||
The goal | To provide students basic knowledge related to operational principles of electrochemical biosensors, with emphasis on practical application in food chemistry, analysis of biological contaminants and application in pharmaceutical industry. | |||
The outcome | Students would adopt knowledge on operational principles of electrochemical biosensors and their application. They would be introduced to development of the specific immobilization techniques of receptor components of the biosensor and basic parameters influencing biosensor function. Student would also be introduced to classification of electrochemical sensors (potentiometric, amperometric, conductometric and impedimetric). They would understand the term and means of formation of enzyme electrodes and be informed on contemporary trends in application of newly developed materials for the fabrication of enzyme electrodes. | |||
Contents | ||||
Contents of lectures | Operational principles of electrochemical biosensors. Major properties (selectivity, range, the nature of the output signal, repeatability and reproducibility, response time, storage time) Enzymes in biosensors, basic structural characteristics. The methods of immobilization of the biomaterial for receptor component (enzymes, antibodies, cells). The influence of the immobilization parameters and techniques on electrochemical biosensors. Novel trends in immobilization and application of: membranes, mediators, modified electrodes for improvement of the electrochemical biosensors function. Mono enzime sensors and sensors with coupled enzymes. Selectivity and possible interference with other bioactive components. Classification of the electrochemical biosensors based on nature of the output signal. Enzyme and non enzyme sensors. Enzyme electrodes. Electrochemical biosensors based on antibodies. The development of microbe sensors and their application. Trends in development of nanostructured materials for fabrication of sensors electrodes. ; Practical aspects of electrochemical biosensors: detection of pesticides, glucose, hydrogen peroxide, urea, ascorbic acid, immuno-sensors. ; | |||
Contents of exercises | Immobilization of enzymes onto materials used in fabrication of sensor electrodes and efficeiency of the immobilization ; The influence of electrode pretreatment on quality of the output signal of non enzymatic electrochemical biosensor; ; Electrochemical determination of ascorbic acid ; Determination of the enzyme kinetic parameters (ex. enzyme electrode for determination of glucose). ; | |||
Literature | ||||
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Number of hours per week during the semester/trimester/year | ||||
Lectures | Exercises | OTC | Study and Research | Other classes |
2 | 1 | 1 | ||
Methods of teaching | Lectures (PowerPoint presentations) and laboratory experiments. Consultations, seminars encompassing relevant literature and discussion of the selected problems. | |||
Knowledge score (maximum points 100) | ||||
Pre obligations | Points | Final exam | Points | |
Activites during lectures | Test paper | 50 | ||
Practical lessons | 10 | Oral examination | ||
Projects | ||||
Colloquia | 20 | |||
Seminars | 20 |